P-225: Gene Variations of Toll-Like Receptor 3 in Endometriosis

Background: Endometriosis is a common gynecologic disorder that is characterized by the ectopic growth of endometrial tissue. Recently, endometriosis has been alternatively described as an immune, genetic and hormonal disease caused by exposure to environmental factors. Toll-like receptor 3 (TLR3) comprises a family of receptors through directly recognizing exogenous and endogenous ligands plays the key role in realization of immune responses, and also involves in the processes of cell proliferation, survival, apoptosis, angiogenesis, tissue remodeling, and repair. Polymorphisms in TLR3 may shift balance between pro- and anti-inflammatory cytokines, modulating the risk of infection, chronic inflammation and cancer. Materials and Methods: We performed a case-control study of 70 endometriosis patients whom had been confirmed by laparoscopic surgery and 55 healthy controls that had no history of inflammatory disorders or using any related drugs. All DNA samples were obtained from Royan DNA bank that had been extracted from peripheral blood along 2012-14. Exon4 of TLR3 were amplified in two overlapped fragments by polymerase chain reaction (PCR) and products were analyzed by sequencing. Results: In fragment1, Missense Single Nucleotide Polymorphism (SNP), rs3775291 (C/T), was observed in 22 out of 70 patients (31.4%) and 24 out of 55 controls (43.7%). Thirty six patients (51.4%) and 31 controls (56.4%) also had shown synonymous SNP,rs3775290 (C/T) in fragment1, while no SNP was observed in fragment2 in 100 cases. The present study showed that the SNPs frequencies between patients and controls, were not significantly different. Conclusion: Endometriosis is a common type of chronic inflammatory disease and both of these SNPs are very common in inflammatory disorders, but these were not significantly different between patient and control group in our studied population.